7U3S

[2T7+21] Self-assembling tensegrity triangle with two turns of DNA and the sticky end addition of a two-turn linker per axis with R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	212.728, 212.728, 174.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.010 - 9.500
R-factor	0.1861
Rwork	0.186
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.011
RMSD bond angle	1.153
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.491	81.491	10.635
High resolution limit [Å]	9.110	19.983	9.110
Number of reflections	1413	202	201
<I/σ(I)>	7.6
Completeness [%]	83.0	99.5
Redundancy	10.9	11.3	11.7
CC(1/2)	0.999	0.999	0.088

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	100 mM MOPS, 125 mM magnesium sulfate, sodium hydroxide