7U3R

[2T7+10] Self-assembling tensegrity triangle with two turns of DNA and the sticky end attachment of a one-turn linker per axis, with R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	157.548, 157.548, 133.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.410 - 6.270
R-factor	0.1209
Rwork	0.118
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.007
RMSD bond angle	1.004
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.410	95.410	7.135
High resolution limit [Å]	6.270	14.912	6.270
Number of reflections	1834	202	203
<I/σ(I)>	12.6
Completeness [%]	85.0	100	51.5
Redundancy	10.9
CC(1/2)	1.000	0.999	0.089

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	120 mM Tris, 60 mM acetic acid, 6 mM EDTA, 150 mM ammonium sulfate, 37.5 mM magnesium sulfate