7U3Q
[4T7] Self-assembling tensegrity triangle with four turns of DNA per axis with R3 symmetry
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-18 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | H 3 |
Unit cell lengths | 205.696, 205.696, 197.198 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.060 - 9.320 |
R-factor | 0.095 |
Rwork | 0.095 |
R-free | 0.10020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbi |
RMSD bond length | 0.015 |
RMSD bond angle | 1.272 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.266 | 86.266 | 10.244 |
High resolution limit [Å] | 9.319 | 20.325 | 9.319 |
Number of reflections | 1708 | 188 | 188 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 86.0 | ||
Redundancy | 10.7 | 10.1 | 11.8 |
CC(1/2) | 1.000 | 1.000 | 0.124 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100 mM MOPS, 125 mM magnesium sulfate, sodium hydroxide |