7U3P

[3T7] Self-assembling tensegrity triangle with three turns of DNA per axis with R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-12
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	159.208, 159.208, 127.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.550 - 6.060
R-factor	0.1303
Rwork	0.128
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.026
RMSD bond angle	2.336
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.705	93.705	6.814
High resolution limit [Å]	6.060	14.691	6.060
Rpim			5.568
Number of reflections	2042	203	212
<I/σ(I)>	8
Completeness [%]	84.7
Redundancy	10.7	10.9	10.9
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	100 mM MOPS, 125 mM magnesium sulfate