7U3D

Structure of S. venezuelae GlgX-c-di-GMP-acarbose complex (4.6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	94.881, 145.379, 229.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.410 - 2.400
R-factor	0.1731
Rwork	0.172
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7u3b
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.530
High resolution limit [Å]	2.400	2.400
Rpim	0.070	0.047
Number of reflections	124127	8764
<I/σ(I)>	11	1.9
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.995	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 300, 100 mM NaCl and 0.1 sodium acetate pH 4.6