7U3B

Structure of S. venezuelae GlgX bound to c-di-GMP and acarbose (pH 8.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-12
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	109.839, 185.588, 184.671
Unit cell angles	90.00, 98.08, 90.00

Refinement procedure

Resolution	48.500 - 3.600
R-factor	0.2383
Rwork	0.237
R-free	0.30750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7u3a
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	3.780
High resolution limit [Å]	3.600	3.600
Rpim	0.170	0.517
Number of reflections	83324	5752
<I/σ(I)>	5.5	1.3
Completeness [%]	98.2
Redundancy	1.7
CC(1/2)	0.923	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% MPD, 0.1 M Tris pH 8.5 and 200 mM ammonium phosphate monobasic