7U2I

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.170, 450.980, 624.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.740 - 2.550
R-factor	0.2251
Rwork	0.223
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	153.740	153.740	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.172	0.057	1.506
Rmeas	0.197	0.065	1.736
Total number of observations	7719767
Number of reflections	1887449	22213	139550
<I/σ(I)>	6.19	17.35	0.93
Completeness [%]	99.4	99.3	99.8
Redundancy	4.09	4.309	3.962
CC(1/2)	0.988	0.993	0.189

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME