7U0K
IOMA class antibody Fab ACS124
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.452, 66.703, 101.939 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.980 - 1.730 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f7e |
RMSD bond length | 0.011 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.980 | 1.740 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.155 | 1.790 |
Rmeas | 0.162 | 1.870 |
Rpim | 0.046 | 0.526 |
Number of reflections | 46469 | 2300 |
<I/σ(I)> | 36.3 | 1.3 |
Completeness [%] | 98.9 | 99.7 |
Redundancy | 12 | 11.9 |
CC(1/2) | 0.890 | 0.470 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M of CHES (pH 9.5) and 38% (v/v) polyethylene glycol 600 |