7TZB

Crystal structure of the human mitochondrial seryl-tRNA synthetase (mt SerRS) bound with a seryl-adenylate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	150.590, 150.590, 142.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.650 - 2.950
R-factor	0.2398
Rwork	0.237
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1WLE
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.650	37.650	3.020
High resolution limit [Å]	2.950	13.180	2.950
Rmerge	0.057	0.032	1.082
Rmeas	0.064	0.036	1.211
Total number of observations	119402
Number of reflections	23497	273	1872
<I/σ(I)>	15.44	39.59	1.63
Completeness [%]	92.4	94.1	99.7
Redundancy	5.082	5.029	4.957
CC(1/2)	0.999	0.999	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9	296	0.1 M Bicine, 10 mM Spermidine, 8% PEG 6000