7TZ1

Crystal Structure of a Mycobacteriophage Cluster A2 Immunity Repressor:DNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979690
Spacegroup name	C 1 2 1
Unit cell lengths	131.267, 44.170, 89.448
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	46.830 - 2.790
R-factor	0.2033
Rwork	0.199
R-free	0.24520
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.776
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.830	2.890
High resolution limit [Å]	2.790	2.790
Number of reflections	10973	521
<I/σ(I)>	11
Completeness [%]	85.4
Redundancy	1.9
CC(1/2)	0.996	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	5-12% PEG 8,000, 0.1-0.3 M CaCl2, and 0.1M HEPES pH 7.5