7TXC
HIC2 zinc finger domain in complex with the DNA binding motif-2 of the BCL11A enhancer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.237, 114.237, 59.146 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.940 - 3.040 |
| R-factor | 0.1934 |
| Rwork | 0.193 |
| R-free | 0.20930 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.940 | 3.160 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.148 | 0.819 |
| Rpim | 0.048 | 0.299 |
| Number of reflections | 16302 | 1640 |
| <I/σ(I)> | 13.5 | 2.5 |
| Completeness [%] | 98.6 | 99.4 |
| Redundancy | 10.1 | 7.8 |
| CC(1/2) | 0.992 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 18% Tacsimate pH7.0 |
