7TW9

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to Sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 65
Unit cell lengths	109.028, 109.028, 48.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.290 - 1.410
R-factor	0.1943
Rwork	0.192
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c8t 7n1o
RMSD bond length	0.008
RMSD bond angle	0.794
Data reduction software	DIALS (2.2.10)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.520	1.600
High resolution limit [Å]	1.410	1.410
Rmerge	0.108	1.190
Rmeas	0.112
Rpim	0.049	0.729
Number of reflections	47235	1275
<I/σ(I)>	9.2
Completeness [%]	90.0	71.3
Redundancy	11.1	7.1
CC(1/2)	0.996	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	11-17% Reagent Alcohol, 0.1-0.3M Lithium Sulfate, and 0.1M Sodium Citrate pH 5.5