7TVH

Hyperlytic variant of Tae1, Type VI secretion amidase effector 1, from Pseudomonas aeruginosa (Cys110Ser)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	91
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97954
Spacegroup name	P 1 21 1
Unit cell lengths	39.121, 108.512, 82.402
Unit cell angles	90.00, 93.23, 90.00

Refinement procedure

Resolution	82.270 - 1.710
R-factor	0.2121
Rwork	0.211
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eob
RMSD bond length	0.006
RMSD bond angle	0.760
Data reduction software	ELVES
Data scaling software	ELVES
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.270	1.771
High resolution limit [Å]	1.710	1.710
Rmerge		0.902
Number of reflections	72921	6437
<I/σ(I)>	10.48
Completeness [%]	98.5
Redundancy	2
CC(1/2)	0.999	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium thiocyanate 0.1 M HEPES pH 7.5 20% PEG3350