7TV9
HUMAN COMPLEMENT COMPONENT C3B IN COMPLEX WITH APL-1030
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.481, 104.699, 103.115 |
| Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
| Resolution | 98.830 - 3.400 |
| R-factor | 0.2436 |
| Rwork | 0.239 |
| R-free | 0.32750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i07 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.830 | 98.640 | 3.610 |
| High resolution limit [Å] | 3.400 | 10.200 | 3.400 |
| Rmerge | 0.154 | 0.029 | 1.492 |
| Rmeas | 0.185 | 0.034 | 1.804 |
| Rpim | 0.102 | 0.019 | 1.003 |
| Total number of observations | 3400 | 14292 | |
| Number of reflections | 28812 | 1111 | 4576 |
| <I/σ(I)> | 6.3 | 25.8 | 0.9 |
| Completeness [%] | 99.2 | 98.4 | 98.1 |
| Redundancy | 3.2 | 3.1 | 3.1 |
| CC(1/2) | 0.994 | 0.998 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 100 mM HEPES, pH 7.8, 25% Poly(acrylic acid sodium salt) 5100 |
