7TV9

HUMAN COMPLEMENT COMPONENT C3B IN COMPLEX WITH APL-1030

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	99.481, 104.699, 103.115
Unit cell angles	90.00, 97.46, 90.00

Refinement procedure

Resolution	98.830 - 3.400
R-factor	0.2436
Rwork	0.239
R-free	0.32750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i07
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.830	98.640	3.610
High resolution limit [Å]	3.400	10.200	3.400
Rmerge	0.154	0.029	1.492
Rmeas	0.185	0.034	1.804
Rpim	0.102	0.019	1.003
Total number of observations		3400	14292
Number of reflections	28812	1111	4576
<I/σ(I)>	6.3	25.8	0.9
Completeness [%]	99.2	98.4	98.1
Redundancy	3.2	3.1	3.1
CC(1/2)	0.994	0.998	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	100 mM HEPES, pH 7.8, 25% Poly(acrylic acid sodium salt) 5100