7TUO

Crystal structure analysis of human USP28 complex with a compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 3
Unit cell lengths	114.640, 114.640, 114.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.060 - 1.960
R-factor	0.1791
Rwork	0.178
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6heh
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.060	81.130	1.990
High resolution limit [Å]	1.960	5.320	1.960
Rmeas	0.112	0.035	2.224
Rpim	0.030	0.010	0.594
Total number of observations	496809	24197	25005
Number of reflections	36300	1942	1792
<I/σ(I)>	17.3	55.5	1.4
Completeness [%]	100.0	100	99.9
Redundancy	13.7	12.5	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.4M Sodium maleic acid, pH 8.0