7TRI

Human antibody S8V1-172 in complex with the influenza hemagglutinin head domain of A/Sydney/05/1997(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 32 2 1
Unit cell lengths	94.700, 94.700, 248.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.560 - 3.600
R-factor	0.2347
Rwork	0.233
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xpz 6mhr 6e4x
RMSD bond length	0.003
RMSD bond angle	0.605
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	3.729
High resolution limit [Å]	3.600	3.600
Rmerge	0.046	0.323
Number of reflections	15613	1543
<I/σ(I)>	12.72
Completeness [%]	99.5	99.87
Redundancy	2	2
CC(1/2)	0.996	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.1 M tris(hydroxymethyl)aminomethane pH 8.5 and 25% (w/v) poly(ethylene glycol) 3350