7TRH

Human antibody K03.28 in complex with the influenza hemagglutinin head domain of A/California/07/2009(H1N1)(X-181)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979100
Spacegroup name	P 1 21 1
Unit cell lengths	75.830, 43.650, 96.910
Unit cell angles	90.00, 105.03, 90.00

Refinement procedure

Resolution	46.800 - 3.000
R-factor	0.2038
Rwork	0.202
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ube 6e56 4hk0 4wuk
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.040	0.382
Number of reflections	24401	2401
<I/σ(I)>	14.93
Completeness [%]	98.3
Redundancy	2
CC(1/2)	0.998	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	25% (w/v) poly(ethylene glycol) 1500