7TR4

MA2-MART1-HLAA0201

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	267.190, 46.498, 78.513
Unit cell angles	90.00, 99.67, 90.00

Refinement procedure

Resolution	46.450 - 2.300
R-factor	0.2254
Rwork	0.223
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gjf
RMSD bond length	0.001
RMSD bond angle	0.461
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.450	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	42458	2605
<I/σ(I)>	9.3
Completeness [%]	98.6
Redundancy	6.7
CC(1/2)	0.995	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG-6000 0.1M Tris (pH8) 0.2M MgCl2