7TQG

Crystal Structure of IOMA Fab inferred germline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.672, 66.703, 166.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.630 - 2.070
R-factor	0.2251
Rwork	0.224
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3z
RMSD bond length	0.008
RMSD bond angle	1.002
Data reduction software	MOSFLM (7.4)
Data scaling software	Aimless (7.1.018)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.630	2.150
High resolution limit [Å]	2.070	2.070
Rmerge	0.080	0.580
Rpim	0.050	0.360
Number of reflections	39372	2597
<I/σ(I)>	9.7	2.5
Completeness [%]	99.0	99
Redundancy	6.3	6.6
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (v/v) PEG 2000, 0.1 M Sodium Acetate (pH 4.6)