7TPD

Integrin alpha IIB beta3 complex with EF5154

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97948
Spacegroup name	P 21 21 2
Unit cell lengths	260.180, 144.340, 104.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.320 - 2.600
R-factor	0.1657
Rwork	0.165
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nig
RMSD bond length	0.003
RMSD bond angle	0.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.090	1.100
Number of reflections	121144	11409
<I/σ(I)>	11
Completeness [%]	99.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	11-13% PEG 8000, 0.2 M Ammonium Sulfate, 0.1 M Tris-HCl, pH 8.9