7TLK
Crystal Structure of K-Ras(G12S)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-09-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 3 |
Unit cell lengths | 83.609, 83.609, 41.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.805 - 1.711 |
R-factor | 0.170866697463 |
Rwork | 0.169 |
R-free | 0.20509 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6usz |
RMSD bond length | 0.008 |
RMSD bond angle | 0.998 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.410 | 1.740 |
High resolution limit [Å] | 1.710 | 1.710 |
Number of reflections | 34820 | 1831 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.9 | |
Redundancy | 9.6 | |
CC(1/2) | 0.987 | 0.941 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1 M Tris, 0.2 M CaCl2, 25% PEG 4K |