7TKW
Crystal structure of R14A human Galectin-7 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97952 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.750, 64.950, 70.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.740 - 1.850 |
R-factor | 0.1947 |
Rwork | 0.193 |
R-free | 0.23090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bkz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.046 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.750 | 53.770 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.692 | 0.695 | 0.888 |
Rmeas | 0.724 | 0.728 | 0.927 |
Rpim | 0.208 | 0.212 | 0.264 |
Number of reflections | 21688 | 2333 | 2125 |
<I/σ(I)> | 142.7 | 568.1 | 12.8 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 12.1 | 11.5 | 12.1 |
CC(1/2) | 0.711 | 0.610 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.2 M Sodium chloride, 0.1 M Tris 8.0, 20 % w/v PEG 6000 |