7TJC
VHH Chl-B2 in complex with Chloramphenicol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.219, 66.553, 93.165 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.150 - 1.350 |
R-factor | 0.2015 |
Rwork | 0.200 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bzq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20rc3_4406) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.150 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rpim | 2.400 | 0.398 |
Number of reflections | 59730 | 6000 |
<I/σ(I)> | 9.1 | 1 |
Completeness [%] | 99.7 | 98.2 |
Redundancy | 12.7 | 10.6 |
CC(1/2) | 0.999 | 0.739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 100 mM sodium acetate, 1 M di-ammonium hydrogen phosphate |