1BZQ

COMPLEX OF A DROMEDARY SINGLE-DOMAIN VHH ANTIBODY FRAGMENT WITH RNASE A

Summary for 1BZQ

• Entry DOI: 10.2210/pdb1bzq/pdb
• Descriptor: PROTEIN (RNASE A), PROTEIN (ANTIBODY CAB-RN05), PHOSPHATE ION (3 entities in total)
• Functional Keywords: immunoglubulin, antibody complex, ribonuclease, hydrolase-immune system complex, hydrolase/immune system
• Biological source: Camelus dromedarius (Arabian camel); More
• Cellular location: Secreted: P61823
• Total number of polymer chains: 8
• Total formula weight: 108540.29

Authors

Decanniere, K.,Desmyter, A.,Gahroudhi, M.,Lauwereys, M.,Muyldermans, S.,Wyns, L. (deposition date: 1998-11-03, release date: 1998-11-11, Last modification date: 2023-08-09)

Primary citation

Decanniere, K.,Desmyter, A.,Lauwereys, M.,Ghahroudi, M.A.,Muyldermans, S.,Wyns, L.
A single-domain antibody fragment in complex with RNase A: non-canonical loop structures and nanomolar affinity using two CDR loops.
Structure Fold.Des., 7:361-370, 1999

PubMed Abstract: Camelid serum contains a large fraction of functional heavy-chain antibodies - homodimers of heavy chains without light chains. The variable domains of these heavy-chain antibodies (VHH) have a long complementarity determining region 3 (CDR3) loop that compensates for the absence of the antigen-binding loops of the variable light chains (VL). In the case of the VHH fragment cAb-Lys3, part of the 24 amino acid long CDR3 loop protrudes from the antigen-binding surface and inserts into the active-site cleft of its antigen, rendering cAb-Lys3 a competitive enzyme inhibitor.

PubMed: 10196124
DOI: 10.1016/S0969-2126(99)80049-5

Experimental method

X-RAY DIFFRACTION (2.8 Å)