7TI6
Crystal structure of the wild-type least mutated common ancestor (LMCA) of the HIV-targeting PCT64 antibody lineage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03319 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.083, 64.317, 76.324 |
Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
Resolution | 48.970 - 2.640 |
R-factor | 0.2299 |
Rwork | 0.229 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ca7 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.589 |
Data reduction software | HKL-2000 (2.3.10) |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.640 | 2.640 |
Rpim | 0.110 | 0.870 |
Number of reflections | 15988 | 811 |
<I/σ(I)> | 8.7 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 5.6 |
CC(1/2) | 0.830 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M HEPES (pH 6.5), 12% (w/v) PEG 6000 |