7THY
Structure of Leucine Rich Repeat Kinase 2's ROC domain interacting with the microtubule facing the minus end
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 5.2 |
Number of Particle | 557577 |
Software | RELION,cryoSPARC,ISOLDE,Rosetta,Leginon,CTFFIND |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |