7THY
Structure of Leucine Rich Repeat Kinase 2's ROC domain interacting with the microtubule facing the minus end
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.2 |
| Number of Particle | 557577 |
| Software | RELION,cryoSPARC,ISOLDE,Rosetta,Leginon,CTFFIND |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
