7TH2
Single-domain VHH intrabodies neutralize ricin toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.644, 63.812, 99.298 |
| Unit cell angles | 90.00, 107.12, 90.00 |
Refinement procedure
| Resolution | 47.449 - 1.838 |
| R-factor | 0.1507 |
| Rwork | 0.149 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtc 4lgr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.911 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 4.970 | 1.830 |
| Rmerge | 0.115 | 0.046 | 0.907 |
| Rmeas | 0.126 | 0.051 | 0.991 |
| Rpim | 0.051 | 0.020 | 0.392 |
| Total number of observations | 372877 | ||
| Number of reflections | 61630 | 3179 | 3023 |
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 98.0 | 97.3 | 96.8 |
| Redundancy | 6.1 | 6.1 | 6.1 |
| CC(1/2) | 0.998 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES pH 6.5, 200 mM sodium thiocyanate, 8% dioxane, 1740 mM ammonium sulfate |
