7TE6

Crystal structure of GluN1b-2B ATD complexed to Fab5 anti-GluN2B antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1
Spacegroup name	P 41 21 2
Unit cell lengths	124.922, 124.922, 407.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.984 - 4.550
R-factor	0.2838
Rwork	0.282
R-free	0.32420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5B3J & 3QEL
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.660
High resolution limit [Å]	4.540	4.540
Rmerge	0.156
Number of reflections	17965	1644
<I/σ(I)>	6.2
Completeness [%]	97.5
Redundancy	7.7
CC(1/2)		0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	2.1 M sodium/potassium phosphate, 100 mM lithium sulfate, 100 mM CAPS, pH 10.5, 4% formamide