7TDX

Structure of FOXP3-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97910
Spacegroup name	P 63 2 2
Unit cell lengths	89.454, 89.454, 176.029
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.770 - 3.100
R-factor	0.2986
Rwork	0.298
R-free	0.31480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6el8
RMSD bond length	0.006
RMSD bond angle	1.188
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.400	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	8070	776
<I/σ(I)>	15.1
Completeness [%]	99.7
Redundancy	19
CC(1/2)	0.994	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	0.5 M lithium sulfate, 2% PEG8000