7T7T

Structure of TSK/BRU1 bound to histone H3.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	82.890, 92.330, 209.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.530 - 3.170
R-factor	0.2816
Rwork	0.279
R-free	0.31830
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.530	3.250
High resolution limit [Å]	3.170	3.170
Number of reflections	28135	2036
<I/σ(I)>	19.5
Completeness [%]	99.9
Redundancy	26.1
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294.15	25% 1,2-Propanediol, 20% glycerol, 0.1M sodium potassium phosphate pH 6