7T4W
Crystal Structure of cGMP-dependent Protein Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | DIAMOND |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.000, 102.621, 104.028 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.060 - 2.230 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.000 | 2.581 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.145 | 0.447 |
| Rpim | 0.068 | 0.377 |
| Number of reflections | 28165 | 1488 |
| <I/σ(I)> | 8 | 1.8 |
| Completeness [%] | 90.6 | 52.2 |
| Redundancy | 5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 0.1M SPG pH 4.0 25% PEG1500 |
