7T4V
Crystal Structure of cGMP-dependent Protein Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | DIAMOND |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.232, 103.690, 104.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.160 - 2.280 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.25600 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.540 | 2.603 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.221 | 0.941 |
Rpim | 0.100 | 0.504 |
Number of reflections | 22622 | 1132 |
<I/σ(I)> | 6.5 | 1.5 |
Completeness [%] | 92.9 | 68.4 |
Redundancy | 5.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 289 | 0.1M SPG pH 8.0 25% PEG1500 |