7T2K
Crystal Structure of TEAD2 in a covalent complex with TED-661
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-09-24 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.910, 61.272, 79.738 |
Unit cell angles | 90.00, 117.46, 90.00 |
Refinement procedure
Resolution | 48.160 - 2.340 |
R-factor | 0.2355 |
Rwork | 0.233 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e5g |
RMSD bond length | 0.005 |
RMSD bond angle | 0.915 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.160 | 2.690 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmeas | 0.139 | 1.310 |
Rpim | 0.072 | 0.700 |
Number of reflections | 12555 | 629 |
<I/σ(I)> | 8.9 | 1.2 |
Completeness [%] | 85.5 | 44.6 |
Redundancy | 3.7 | 3.3 |
CC(1/2) | 0.994 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium Formate 1.8 M - 2.4 M Hepes pH 7.2 - 7.4 |