7T18

Rev1 Ternary Complex with dTTP and Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-09
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	62.202, 73.639, 117.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.660 - 1.700
R-factor	0.1838
Rwork	0.182
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wm1
RMSD bond length	0.008
RMSD bond angle	0.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.660	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	60303	2977
<I/σ(I)>	15.06
Completeness [%]	99.8
Redundancy	4.3
CC(1/2)	0.999	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15-23% PEG3350, 200 mM ammonium nitrate