7T0Y
The Ribosomal RNA Processing 1B Protein Phosphatase-1 Holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.528, 160.387, 50.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.270 - 1.800 |
R-factor | 0.2093 |
Rwork | 0.208 |
R-free | 0.24620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mov |
RMSD bond length | 0.005 |
RMSD bond angle | 0.818 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.19.1_4158) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.207 | 1.280 |
Number of reflections | 61295 | 4407 |
<I/σ(I)> | 4.9 | |
Completeness [%] | 96.2 | 94.8 |
Redundancy | 4.6 | 4.6 |
CC(1/2) | 0.981 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% ethylene glycol; 10% PEG8000, 0.1 M imidazole; MES acid, 0.09 M Sodium fluoride; 0.09 M Sodium bromide; 0.09 M Sodium iodide |