7SZB
Crystal Structure Analysis of human PRPK complex with a compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 295.770, 47.630, 62.140 |
Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.710 | 61.740 | 2.050 |
High resolution limit [Å] | 2.020 | 5.480 | 2.020 |
Rmeas | 0.091 | 0.028 | 1.094 |
Rpim | 0.047 | 0.015 | 0.562 |
Total number of observations | 208593 | 10313 | 10568 |
Number of reflections | 56764 | 2960 | 2841 |
<I/σ(I)> | 14.3 | 40.5 | 1.6 |
Completeness [%] | 99.6 | 98.4 | 100 |
Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 100mM Sodium citrate pH 5.2, 12.8% PEG8000, 10mM MgCl2, 10mM TCEP |