7SXH

BIO-8546 bound GSK3beta-axin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	68.610, 72.640, 79.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.290 - 2.090
R-factor	0.2159
Rwork	0.214
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kpl
RMSD bond length	0.003
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.290	2.170
High resolution limit [Å]	2.090	2.090
Number of reflections	23943	2242
<I/σ(I)>	14.65
Completeness [%]	99.3
Redundancy	6.5
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M calcium acetate, 0.1M BisTRIS pH 7.0, 5% Glycerol and 17% PEG3350