7SX7
Crystal structure of broadly neutralizing antibody N49P9.3-FR3-3 Fab in complex with HIV-1 Clade A/E strain 93TH057 gp120 core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 |
Unit cell lengths | 60.367, 65.418, 112.381 |
Unit cell angles | 90.02, 104.85, 90.02 |
Refinement procedure
Resolution | 46.660 - 2.150 |
R-factor | 0.2116 |
Rwork | 0.209 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6oz3 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.946 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.135 | 0.645 |
Rpim | 0.135 | 0.645 |
Number of reflections | 73767 | 2786 |
<I/σ(I)> | 6.9 | 1 |
Completeness [%] | 82.7 | 63.6 |
Redundancy | 1.8 | 1.4 |
CC(1/2) | 0.949 | 0.394 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 10% PEG 3350 0.1 M HEPES pH 7.0 0.1 M magnesium chloride |