7SWA
MicroED structure of proteinase K from a 320 nm thick lamella measured at 300 kV
Experimental procedure
Collection date | 2020-10-01 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.340, 68.340, 105.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.370 - 2.100 |
R-factor | 0.2015 |
Rwork | 0.199 |
R-free | 0.24460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cl7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.299 | 1.210 |
Rmeas | 0.357 | 1.441 |
Rpim | 0.144 | |
Number of reflections | 71235 | 5348 |
<I/σ(I)> | 3.61 | 1.02 |
Completeness [%] | 82.5 | 83.3 |
Redundancy | 3.1 | |
CC(1/2) | 0.964 | 0.347 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |