7SWA

MicroED structure of proteinase K from a 320 nm thick lamella measured at 300 kV

Experimental procedure

Collection date	2020-10-01
Spacegroup name	P 43 21 2
Unit cell lengths	68.340, 68.340, 105.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.370 - 2.100
R-factor	0.2015
Rwork	0.199
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.006
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.299	1.210
Rmeas	0.357	1.441
Rpim	0.144
Number of reflections	71235	5348
<I/σ(I)>	3.61	1.02
Completeness [%]	82.5	83.3
Redundancy	3.1
CC(1/2)	0.964	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
1