7SV9

Structure of EmrE-D3 mutant in complex with monobody L10 and TPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9183
Spacegroup name	C 1 2 1
Unit cell lengths	140.710, 50.141, 110.282
Unit cell angles	90.00, 93.45, 90.00

Refinement procedure

Resolution	55.040 - 3.360
R-factor	0.2873
Rwork	0.286
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wk8
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.228	3.620
High resolution limit [Å]	3.360	3.360
Rmerge	0.340	1.053
Rmeas	0.384	1.150
Number of reflections	6130	307
<I/σ(I)>	4
Completeness [%]	84.1
Redundancy	5.9
CC(1/2)	0.779	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	0.1 M lithium nitrate, 0.1 M ADA, pH 6.5, 32% PEG600