7SUE
Crystal Structure of Human Fab S24-188 in the complex with the N-teminal Domain of Nucleocapsid protein from SARS CoV-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-03 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 1 |
Unit cell lengths | 71.310, 78.500, 127.150 |
Unit cell angles | 97.60, 89.96, 90.02 |
Refinement procedure
Resolution | 39.200 - 2.900 |
R-factor | 0.264 |
Rwork | 0.262 |
R-free | 0.29190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | swiss model |
RMSD bond length | 0.003 |
RMSD bond angle | 0.635 |
Data reduction software | xia2 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.085 | 1.428 |
Number of reflections | 55485 | 2773 |
<I/σ(I)> | 8.6 | 0.9 |
Completeness [%] | 91.6 | 92.2 |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.997 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1 M Sodium Citrate pH 5.6, 20 % (w/v) PEG 4000, 20 % (v/v) 2-propanol |