7SSE
Crystal structure of the WDR domain of human DCAF1 in complex with CYCA-117-70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.956, 87.919, 73.878 |
| Unit cell angles | 90.00, 98.72, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.620 |
| R-factor | 0.2069 |
| Rwork | 0.206 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pxw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 4.400 | 1.620 |
| Rmerge | 0.041 | 0.030 | 0.516 |
| Rmeas | 0.048 | 0.035 | 0.651 |
| Rpim | 0.024 | 0.018 | 0.390 |
| Total number of observations | 269845 | ||
| Number of reflections | 76489 | 3953 | 3068 |
| <I/σ(I)> | 13.5 | ||
| Completeness [%] | 97.4 | 98.4 | 78.4 |
| Redundancy | 3.5 | 3.7 | 2.1 |
| CC(1/2) | 0.997 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 30% PEG3350 ,0.1M Ammonium Sulphate, 0.1M Bis-Tris pH5.5 |
