7SRV

Metal dependent activation of Plasmodium falciparum M17 aminopeptidase (inactive form), spacegroup P22121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953724
Spacegroup name	P 2 21 21
Unit cell lengths	111.542, 172.670, 179.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.190 - 2.030
R-factor	0.19
Rwork	0.189
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kqz
RMSD bond length	0.002
RMSD bond angle	0.608
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	233061	11469
<I/σ(I)>	7.1
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.997	0.244

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20 % PEG3350, 0.2 M calcium acetate