7SR6

Human Endogenous Retrovirus (HERV-K) reverse transcriptase ternary complex with dsDNA template Primer and dNTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.49993
Spacegroup name	P 31
Unit cell lengths	177.697, 177.697, 117.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	88.848 - 2.620
Rwork	0.168
R-free	0.21840
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.626
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.900	2.710
High resolution limit [Å]	2.620	2.620
Rmerge	0.080	1.080
Rmeas	0.080
Rpim	0.030	0.350
Number of reflections	114382	5724
<I/σ(I)>	17.6	2
Completeness [%]	91.8	49.4
Redundancy	10.7
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	10% PEG400, 50 mM MES pH 6.0, 2 mM MgCl2 and 100 mM KCl)