7SQP

Single chain trimer HLA-A*02:01 (Y108C) with HPV.16 E7 peptide YMLDLQPETTDL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	117.979, 117.979, 262.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 2.530
R-factor	0.2398
Rwork	0.239
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jf1
RMSD bond length	0.002
RMSD bond angle	0.487

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.530	2.530
Number of reflections	61220	5234
<I/σ(I)>	33.9
Completeness [%]	97.9
Redundancy	9.3
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	200 mM NH4Citrate, 20.0% PEG 3350