7SPT
Crystal structure of exofacial state human glucose transporter GLUT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-10-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.231, 118.430, 53.728 |
| Unit cell angles | 90.00, 103.16, 90.00 |
Refinement procedure
| Resolution | 46.965 - 2.100 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zw9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 34213 | 4424 |
| <I/σ(I)> | 6.34 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.49 | |
| CC(1/2) | 0.980 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 40% v/v PEG400, 100 mM Tris, pH 7.6, 100 mM magnesium chloride |
