7SPL
[2T3] Self-assembling 3D DNA triangle with three inter-junction base pairs containing the L1 junction and a zero-linked center strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-21 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | H 3 |
Unit cell lengths | 87.841, 87.841, 140.534 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.720 - 6.090 |
R-factor | 0.236 |
Rwork | 0.234 |
R-free | 0.33480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbi |
RMSD bond length | 0.006 |
RMSD bond angle | 1.301 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 66.900 | 66.900 | 7.264 |
High resolution limit [Å] | 6.033 | 10.615 | 6.033 |
Number of reflections | 765 | 192 | 191 |
<I/σ(I)> | 4.4 | ||
Completeness [%] | 83.2 | 100 | 55.2 |
Redundancy | 9.9 | ||
CC(1/2) | 0.995 | 0.995 | 0.039 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 100 mM MOPS, 1.25 M magnesium sulfate |