7SOR

LaM domain of human LARP1 in complex with AAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.96860
Spacegroup name	P 21 21 21
Unit cell lengths	48.562, 55.890, 70.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.720 - 1.350
R-factor	0.1819
Rwork	0.181
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.014
RMSD bond angle	1.584
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	42380	4097
<I/σ(I)>	44.5
Completeness [%]	99.8
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium chloride, 0.1M Bis-Tris pH 5.5, 25% PEG3350