7SNQ

Hexamer HIV-1 CA in complex with CPSF6 peptide and IP6 ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-08-08
Detector	RDI CMOS_8M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	133.918, 135.673, 212.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.240 - 2.810
R-factor	0.2315
Rwork	0.229
R-free	0.28170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u0b
RMSD bond length	0.009
RMSD bond angle	1.076
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.220	2.860
High resolution limit [Å]	2.810	2.810
Rmerge	0.083	1.191
Number of reflections	94907	4661
<I/σ(I)>	16.5	1.4
Completeness [%]	99.9	100
Redundancy	6.4	6.1
CC(1/2)	0.999	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M Tris pH 8.2, 8% peg 8000, 5% Glycerol