7SLS
HIV Reverse Transcriptase with compound Pyr02
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-05 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.728, 154.253, 155.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 93.580 - 2.078 |
R-factor | 0.2282 |
Rwork | 0.227 |
R-free | 0.25850 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.8 (10-DEC-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.586 | 2.224 |
High resolution limit [Å] | 2.078 | 2.078 |
Number of reflections | 69946 | 3498 |
<I/σ(I)> | 21.6 | |
Completeness [%] | 82.2 | |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 950 mM sodium malonate pH 7.0, 100 mM HEPES/NaOH pH 6.8 |